Faculty of Philosophy Sciences and Letters of Ribeirão Preto
University of Sao Paulo
Research lines
Our research group has been developing chromatographic and electrophoretic methods (capillary electrophoresis) for the analysis of different analytes in different matrices. The main lines of research that we have been working on are listed below
Evaluation of the Effects of Emerging Contaminants on CYP450 Enzymes
This research line focuses on the development and validation of chromatographic methods to investigate the in vitro metabolism and inhibition of CYP450 enzymes, with an emphasis on interactions between emerging contaminants and therapeutic drugs. Using chromatographic techniques and human liver microsomes, our goal is to determine how these xenobiotics are metabolized and how they interfere with CYP450 activity, which plays a central role in the metabolism of many pharmaceuticals. Such interactions may alter drug efficacy or increase toxicity, making it essential to predict these effects to ensure safety under combined exposure scenarios
Miniaturized sample preparation techniques
Development of new miniaturized sample preparation procedures for the analysis of drugs and metabolites in different matrices (plasma, urine, culture medium, liver microsomes) using chromatographic or electrophoretic techniques. The main techniques developed are solid phase microextraction (SPME), liquid phase microextraction using hollow cylindrical membranes (HF-LPME) and dispersive liquid-liquid microextraction (DLLME).
Enantioselective analysis of pharmacologically active metabolites using fungi in the biotransformation process
Development and validation methods for the enantioselective analysis of pharmacologically active and chiral metabolites using fungi in the biotransformation process and suggest a new way of obtaining such metabolites. The analytical techniques employed are: high-performance liquid chromatography and capillary electrophoresis.
