Laboratory of in Vitro Metabolism and Separation Techniques

*PEROVANI, I. S.; BARBETTA, M. F. S.; SILVA, R. M.; LOPES, N. P.; de OLIVEIRA, A. R. M. In vitro-in vivo correlation of the chiral pesticide prothioconazole after interaction with human CYP450 enzymes. Food and Chemical Toxicology, v. 163, p. 112947, 2022. doi: https://doi.org/10.1016/j.fct.2022.112947

 

*COSTA, E. M. A.; DE ANDRADE FIGUEIRA, M. B.; CARRÃO, D. B.; HABENSCHUS, M. D.; DE OLIVEIRA, A. R. M.; ACHO, L. D. R.; LIMA, E. S.; DOS SANTOS MAGALHÃES, I. R. Phenolic and flavonoid content and in vitro inhibitory effect of some Amazonian fruit juices on CYP3A4 activity. Acta Amazonica, v. 52, p. 172-178, 2022. doi: https://doi.org/10.1590/1809-4392202103642

 

*TADINI, M. C.; FERNANDES, F. S.; OZELIN, S. D.; DE MELO, M. R. S.; MANSUR, A. L.; TOLEDO, T. B.; DE ALBUQUERQUE, N. C. P.; TAVARES, D. C.; MARQUELE-OLIVEIRA, F. ; DE OLIVEIRA, A. R. M. Pharmacokinetic study of AmB-NP-GR: a new granule form with amphotericin B to treat leishmaniasis and fungal infections. European Journal of Pharmaceutical Sciences, v. 173, p. 106173, 2022. doi: https://doi.org/10.1016/j.ejps.2022.106173 - open access

 

*TADINI, M. C.; BALLESTERO, G.; PEROVANI, I. S.; ALBUQUERQUE, N. C. P. ; FORTE, A. L. A.; MARQUELE-OLIVEIRA, F.; de OLIVEIRA, A. R. M. Predicting absorption of amphotericin B encapsulated in a new delivery system by an in vitro Caco-2 cell model. Journal of Drug Delivery Science and Technology, v. 71, p. 103345, 2022. doi: https://doi.org/10.1016/j.jddst.2022.103345 

 

*HABENSCHUS, M. D.; CARRAO, D. B.; ALBUQUERQUE, N. C. P.; PEROVANI, I. S.; SILVA, R. M.; NARDINI, V.; LOPES, N. P.; DIAS, L. G.; de OLIVEIRA, A. R. M. In vitro enantioselective inhibition of the main human CYP450 enzymes involved in drug metabolism by the chiral pesticide tebuconazole. Toxicology Letters, v. 351, p. 1-9, 2021. doi: https://doi.org/10.1016/j.toxlet.2021.08.006

 

*PEROVANI, I. S.; BUCCI, J. L. M.; CARRAO, D. B.; BARBETTA, M. F. S.; SILVA, R. M.; LOPES, N. P.; de OLIVEIRA, A. R. M. Enantioselective inhibition of human CYP2C19 by the chiral pesticide ethofumesate: Prediction of pesticide-drug interactions in humans. Chemico-Biological Interactions, v. 345, p. 109552, 2021. doi: https://doi.org/10.1016/j.cbi.2021.109552

 

* PEROVANI, IS; SERPELLONE, CO; de OLIVEIRA, ARM An appraisal of experimental designs: application to enantioselective capillary electromigration techniques. Electrophoresis , 2021. doi: https://doi.org/10.1002/elps.202000334

 

* PEROVANI, IS; CARRAO, DB; ALBUQUERQUE, NCP; de OLIVEIRA, ARM Enantioselective in vitro metabolism and in vitro-in vivo correlation of the herbicide ethofumesate in a human model. Journal of Pharmaceutical and Biomedical Analysis , 2020. doi: https://doi.org/10.1016/j.jpba.2020.113349

 

* CARRAO, DB; PEROVANI, IS; ALBUQUERQUE, NCP ; de OLIVEIRA, ARM Enantioseparation of pesticides: A critical review. TRAC-Trends in Analytical Chemistry , v. 122, p. 115719, 2020. doi: https://doi.org/10.1016/j.trac.2019.115719

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* ALBUQUERQUE, NCP; CARRAO, DB; HABENSCHUS, MD; FONSECA, FS; SILVA, RM; LOPES, NP; ROCHA, BA; BARBOSA JUNIOR, F .; de OLIVEIRA, ARM Risk assessment of the chiral pesticide fenamiphos in a human model: Cytochrome P450 phenotyping and inhibition studies. Food and Chemical Toxicology , v. 146, p. 111826, 2020. doi: https://doi.org/10.1016/j.fct.2020.111826

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* SILVA, RM; CARRÃO, DB; HABENSCHUS, MD; JIMENEZ, P .; LOPES, NP; FENICAL, W .; COSTA-LOTUFO, L .; de OLIVEIRA, ARM Prediction of seriniquinone-drug interactions by in vitro inhibition of human cytochrome P450 enzymes. Toxicology in Vitro , v. 65, p. 104820, 2020. doi: https://doi.org/10.1016/j.tiv.2020.104820

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* JANG, I .; CARRÃO, D. B; MENGER, R .; by OLIVEIRA, ARM; HENRY, C. Pump-Free Microfluidic Rapid Mixer Combined with a Paper-Based Channel. ACS Sensors , v. 5, p. 2230-2238, 2020. doi: https://doi.org/10.1021/acssensors.0c00937

 

* MARTINS, J.; TADINI, MC; SOUZA, RO; FIGUEIREDO, SA; FONSECA, MJV; ALBUQUERQUE, NCP; LOPES, NP; BERRETTA, AA; Marquele-Oliveira, F.; from OLIVEIRA, ARM. Yangambin and Epi-yangambin Isomers: New Purification Method from Ocotea fasciculata and First Cytotoxic Aspects Focusing on In Vivo Safety. Medical Plant , v. 86, p. 415-424, 2020. doi: https://doi.org/10.1055/a-1118-3828

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* CARRAO, DB; HABENSCHUS, MD ; ALBUQUERQUE, NCP ; SILVA, RM; LOPES, NP ; de OLIVEIRA, ARM In vitro inhibition of human CYP2D6 by the chiral pesticide fipronil and its metabolite fipronil sulfone: Prediction of pesticide-drug interactions. Toxicology Letters , v. 313, p. 196-204, 2019. doi: https://doi.org/10.1016/j.toxlet.2019.07.005

 

* FONSECA, FS; CARRAO, DB; ALBUQUERQUE, NCP; NARDINI, V .; DIAS, LG; SILVA, RM; LOPES, NP; de OLIVEIRA, ARM Myclobutanil enantioselective risk assessment in humans through in vitro CYP450 reactions: Metabolism and inhibition studies. Food and Chemical Toxicology. v. 128, p. 202-211,   2019. doi: https://doi.org/10.1016/j.fct.2019.04.009

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* HABENSCHUS, MD ; NARDINI, V .; DIAS, LG; ROCHA, BA ; BARBOSA JUNIOR, F .; de OLIVEIRA, ARM In vitro enantioselective study of the toxicokinetic effects of chiral fungicide tebuconazole in human liver microsomes. Ecotoxicology and Environmental Safety, v. 181, p. 96-105, 2019. doi: https://doi.org/10.1016/j.ecoenv.2019.05.071

 

* PEROVANI, IS; de OLIVEIRA, ARM Determination of ethofumesate-2-hydroxy stereolability by dynamic HPLC. Separation Science Plus , 2018. doi: http://dx.doi.org/10.1002/sscp.201800112

 

* SILVA, R. M .; Gaitani C. M .; Marques, LM M .; BARACO , K. F .; CAVALHEIRO, A. J .; MORAES, LA B .; LOPES, N. P .; de OLIVEIRA, AR M. Characterization of casearin X metabolism by rat and human liver microsomes. Medical Plant , v. 85, p. 282-291, 2019. doi: https://doi.org/ 10.1055 / a-0765-95232018

 

* CARRAO, D. B .; GOMES, ICD R .; BARBOSA JUNIOR, F .; de OLIVEIRA, AR M.. Evaluation of the enantioselective in vitro metabolism of the chiral pesticide fipronil employing a human model: Risk assessment through in vitro-in vivo correlation and prediction of toxicokinetic parameters. Food and Chemical Toxicology , 2018. doi: https://doi.org/10.1016/j.fct.2018.10.060

 

* MOREIRA, FL; RIUL, TB; MOREIRA, ML; PILON, AC; DIAS-BARUFFI, M .; ARAUJO, MSS; LOPES, NP; from OLIVEIRA, ARM. Leishmanicidal Effects of Piperlongumine (Piplartine) and Its Putative Metabolites. Planta Medica , 2018. doi: https://doi.org/ 10.1055 / a-0614-2680

 

* ALBUQUERQUE, NC P; CARRÃO, DB; HABENSCHUS, MD Metabolism studies of chiral pesticides: A critical review. Journal of Pharmaceutical and Biomedical Analysis, v. 147, p.89-109, 2018. doi: https://doi.org/10.1016/j.jpba.2017.08.011

 

* HABENSCHUS, MD ; MOREIRA, FL ; LOPES, NP ; de OLIVEIRA, ARM In Vitro Inhibition of Human CYP450s 1A2, 2C9, 3A4 / 5, 2D6 and 2E1 by Grandisin. Planta Medica , 2017. doi: http://dx.doi.org/10.1055/s-0042-124615

 

* CARRAO, DB; ALBUQUERQUE, NCP ; MARQUES, LMM ; CROTTI, AEM; PILON, AC; BOLZANI, VS; BERRETTA, AA; de OLIVEIRA, ARM In Vitro Metabolism of Artepillin C by Rat and Human Liver Microsomes. Planta Medica , 2017. doi: http://dx.doi.org/10.1055/s-0042-124359

 

* MOREIRA, FL; MARQUES, LMM; LOPES, NP; de OLIVEIRA, ARM In vitro metabolism studies of natural products are crucial in the early development of medicines. Phytochemistry Reviews, v. 16, p. 355-373, 2017 . doi: 10.1007 / s11101-016-9482-0

 

* MOREIRA, FL; HABENSCHUS, MD; BARTH, T.; Marques, LMM; PILON, AC; BOLZANI, VS; VESSECCHI, R.; LOPES, NP; de OLIVEIRA, ARM Metabolic profile and safety of piperlongumine. Scientific Reports , v. 6, p. 33646, 2016. doi: 10.1038 / srep33646   - open access

 

* ALBUQUERQUE, NCP ; MATOS, JV; de OLIVEIRA, ARM In-line coupling of an achiral-chiral column to investigate the enantioselective in vitro metabolism of the pesticide Fenamiphos by human liver microsomes. Journal of Chromatography A , v. 1467, p. 326-334, 2016. doi: http://dx.doi.org/10.1016/j.chroma.2016.08.039

 

* Bocato, MZ; Moreira, FL; Albuquerque, NCP; from Gaitani, CM; de Oliveira, ARM In vitro enantioselective human liver microsomal metabolism and prediction of in vivo pharmacokinetic parameters of tetrabenazine by DLLME-CE. Journal of Pharmaceutical and Biomedical Analysis , v. 128, p. 528-537, 2016. doi: 10.1016 / j.jpba.2016.06.028

 

* BARTH, T .; HABENSCHUS, MD; MOREIRA, FL; FERREIRA, LS; LOPES, NP; de OLIVEIRA, ARM In vitro metabolism of the lignan (-) - grandisin, an anticancer drug candidate, by human liver microsomes. Drug Testing Analysis , v. 7, p. 780-786, 2015. doi: 10.1002 / dta.1743

 

* ALBUQUERQUE, NCP; from Gaitani, CM; de OLIVEIRA, ARM A new and fast DLLME-CE method for the enantioselective analysis of zopiclone and its active metabolite after fungal biotransformation. Journal of Pharmaceutical and Biomedical Analysis , v. 109, p. 192-201, 2015. doi: 10.1016 / j.jpba.2015.02.039

 

* BARTH, T .; HILARIO, VC; ROCHA, BA; FURTADO, NAJC; PUPO, MT; de OLIVEIRA, ARM Asymetric Sulfoxidation of Albendazole to Ricobendazole by Fungi: Effect of pH. New Chemistry , v. 38, p. 944-947, 2015. doi.org/10.5935/0100-4042.20150102

 

* BORTOLETO, MA; BOCATO, MZ; PUPO, MT; Gaitani CM; de OLIVEIRA, ARM Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main Metabolites after Biotransformation by Fungi. Journal of the Brazilian Chemical Society , v. 26, p. 1956-1966, 2015. doi.org/10.5935/0103-5053.20150174

 

ROCHA, BA; ASSIS, MD; PETI, APF; MORAES, LAB; MOREIRA, FL; LOPES, NP; POSPIIL, S .; GATES, PJ; de OLIVEIRA, ARM In vitro metabolism of monensin A: microbial and human liver microsomes models. Xenobiotica , v. 44, p. 326-335, 2014. doi: 10.3109 / 00498254.2013.845707

 

* Marques, LMM; SILVA-JUNIOR, EA; GOUVEA, D. R; VESSECCHI, R .; Pupo, MT; LOPES, NP; KATO, MJ; de OLIVEIRA, ARM In vitro metabolism of the alkaloid piplartine by rat liver microsomes. Journal of Pharmaceutical and Biomedical Analysis , v. 95, p. 113-120, 2014. doi: 10.1016 / j.jpba.2014.02.020

 

* ROCHA, BA; by OLIVEIRA, ARM; PAZIN, M .; DORTA, D .; NASCIMENTO, AP; BERRETTA, AA; PETI, APF; MORAES, LAB; LOPES, NP; POSPIIL, S .; GATES, PJ; ASSIS, MD Jacobsen catalyst as a cytochrome P450 biomimetic model for the metabolism of monensin A. BioMed Research International , v. 2014, p. 1-8, 2014. doi.org/10.1155/2014/152102

 

* BOCATO, MZ; BORTOLETO, MA; PUPO, MT; de OLIVEIRA, ARM A new enantioselective CE method for determination of oxcarbazepine and licarbazepine after fungal biotransformation. Electrophoresis , v. 35, p. 2877-2884, 2014. doi: 10.1002 / elps.201400137

 

* MESSIANO, GB; SANTOS, RAS; FERREIRA, LS; Simões, RA; Jabor, VAP; KATO, MJ; LOPES, NP; Pupo, MT; de Oliveira, ARM In vitro metabolism study of the promising anticancer agent the lignan (-) - grandisin. Journal of Pharmaceutical and Biomedical Analysis , v. 72, p. 240-244, 2013. doi: 10.1016 / j.jpba.2012.08.028

 

* MOREIRA, FL; SOUZA, GHB; RODRIGUES, IV; LOPES, NP; de OLIVEIRA, ARM A non-michaelian behavior of the in vitro metabolism of the pentacyclic triterpene alfa and beta amyrins by employing rat liver microsomes. Journal of Pharmaceutical and Biomedical Analysis , v. 84, p. 14-19, 2013. doi: 10.1016 / j.jpba.2013.05.038

 

* Fortes, SS; BARTH, T .; FURTADO, NAJC; Gaitani CM; PUPO, MT; de OLIVEIRA, ARM Evaluation of dispersive liquid-liquid microextraction in the stereoselective determination of cetirizine following the fungal biotransformation of hydroxyzine and analysis by capillary electrophoresis. Talanta , v. 116, p. 743-752, 2013. doi: 10.1016 / j.talanta.2013.07.062

 

* Marques, LMM; de OLIVEIRA, ARM Chromatographic techniques and the challenge in determining compounds present in complex matrices: Report of a practical case of the natural product piperlongumin. Scientia Chromatographica , v. 5, p. 101-109, 2013. doi: org / 10.4322 / sc.2013.008

 

* ALBUQUERQUE, NCP; BORTOLETO, MA; de OLIVEIRA, ARM Enantioselective analysis of drugs and metabolites in biological matrices using miniaturized sample preparation techniques. Scientia Chromatographica , v. 5, p. 214-228, 2013. doi: org / 10.4322 / sc.2014.005

 

* Hilário, VC; Carrão, DB; Barth, T .; Borges, KB; Furtado, NAJC; Pupo, MT; de Oliveira, ARM Assessment of the stereoselective fungal biotransformation of albendazole and its analysis by HPLC in polar organic mode. Journal of Pharmaceutical and Biomedical Analysis , v. 61, p. 100-107, 2012. doi: 10.1016 / j.jpba.2011.12.012

 

* Bocato, MZ; Simões, RA; Calixto, LA; Gaitani, CM; Pupo, MT; Oliveira, ARM Solid phase microextraction and LC-MS / MS for the determination of paliperidone after stereoselective fungal biotransformation of risperidone. Analytica Chimica Acta , v. 742, p. 80-89, 2012. doi: 10.1016 / j.aca.2012.05.056

 

* de Oliveira, ARM Enantioselective analysis of drugs and metabolites using capillary electrophoresis. Scientia Chromatographica , v. 3, p. 231-247, 2011. doi: 10.4322 / sc.2011.014

 

* Car, DB; Borges, KB; Barth, T .; Pupo, MT; Bonato, PS; de Oliveira, ARM Capillary electrophoresis and hollow fiber liquid-phase microextraction for the enantioselective determination of albendazole sulfoxide after biotransformation of albendazole by an endophytic fungus. Electrophoresis , v. 32, p. 2746-2756, 2011. doi: 10.1002 / elps.201000658

 

* from Jesus, LI; Albuquerque, NCP; Borges, KB; Simões, RA; Calixto, LA; Furtado, NAJC; from Gaitani, CM; Pupo, MT; de Oliveira, ARM Enantioselective fungal biotransformation of risperidone in liquid culture medium by capillary electrophoresis and hollow fiber liquid-phase microextraction. Electrophoresis , v. 32, p. 2765-2775, 2011. doi: 10.1002 / elps.201100328

 

* by OLIVEIRA, ARM; Marquele-Oliveira, F .; SANTANA, DCAS; NIKOLAOU, S .; Bonato PS; SILVA, RS HPLC separation, NMR and QTOF / MS / MS structure elucidation of a prominent nitric oxide donor agent based on an isomeric composition of a nitrosyl ruthenium complex. Inorganic Chemistry Communications , v. 12, p. 343-346, 2009. doi: 10.1016 / j.inoche.2009.02.001

 

* by OLIVEIRA, ARM; da FONSECA, P .; CURTI, C .; SILVA, RS; Bonato PS In vitro metabolism study of a new nitrosyl ruthenium complex [RU (NH.NHq) (terpy) NO] 3+ nitric oxide donor using rat microsomes. Nitric Oxide. Biology and Chemistry , v. 21, p. 14-19, 2009. doi: 10.1016 / j.niox.2009.03.003

 

* by OLIVEIRA, ARM; MAGALHAES, IRS; Santana, FJM; Bonato PS Microextraction in liquid phase (LPME): Fundamentals of the technique and applications in the analysis of drugs in biological fluids. New Chemistry , v. 31, p. 637-644, 2008. doi: org / 10.1590 / S0100-40422008000300031

 

* by OLIVEIRA, ARM; CARDOSO, CD; Bonato PS Stereoselective determination of hydroxychloroquine and its metabolites in human urine by liquid-phase microextraction and CE. Electrophoresis , v. 28, p. 1081-1091, 2007. doi: 10.1002 / elps.200600420

 

* by OLIVEIRA, ARM; Bonato PS Stereoselective determination of hydroxychloroquine and its major metabolites in human urine by solid-phase microextraction and HPLC. Journal of Separation Science , Vol. 30, p. 2351-2359, 2007. doi: 10.1002 / jssc.200700121

 

* by OLIVEIRA, ARM; Cesarino, EJ; Bonato PS Solid-phase microextraction and chiral HPLC analysis of ibuprofen in urine. Journal of Chromatography B , v. 818, p. 285-291, 2005. doi: 10.1016 / j.jchromb.2005.01.010

 

* by OLIVEIRA, ARM; Santana, FJM; Bonato PS Stereoselective determination of the major ibuprofen metabolites in human urine by off-line coupling solid-phase microextraction high-performance liquid chromatography. Analytica Chimica Acta , v. 538, p. 25-34, 2005. doi: 10.1016 / j.aca.2005.01.058